I need your assistance:
I am using UCSC's In Silico PCR to test primers that have been designed by a previous student. I should amplify a partial region on chromosome 7 (gene TAS2R38)
The designs are:
F: 5’- CCT TCG TTT TCT TGG TGA ATT TTT GGG ATG TAG TGA AGA GGC GG -3’
R: 5’- AGG TTG GCT TGG TTT GCA ATC ATC -3’
However, when I plug them in, the output suggests that it amplifies a region on chromosome 22. Can anyone explain why I'm getting these results?
Thank you kindly.