problem with TNT coupled invitro transcription/
Posted 16 April 2004 - 06:42 AM
i appriciate it very much
Posted 17 April 2004 - 11:58 AM
You have stated that your control is working fine. There is something in your test sample DNA that is causing the problem. Test this by adding 2 -8ul of heat denatured test sample to the kit control. If you get no band now, you have an inhibitor originating in your test sample.
Good luck and God Bless
Edited by phdconsult, 18 April 2004 - 02:56 PM.