We are busy doing luciferase assays (dual). We would like to do a transient transfection of Jurkat.
Since Promega has a protocol in its database we bought Fugene HD as transfection agent.
It works extremely well on HepG2, but we have absolute zero success with Jurkat.
We tried 1.5:1 -8:1 ratios in +1 steps, tried 3x105 and 5x105 cells, and both 24h incubation and 36h incubation.
We are using IMDM 2mM L-glut, 10%FBS and pen/strep as culture medium.
Does anybody have good tips/protocols to share how to transfect Jurkat with a luciferase vector?
Preferably with Fugene HD, but at this stage we'll settle for anything that works...
We are stuck...