Hi, got a general question: Is there anything equivalent to serum starvation for cells that are regularly kept in serum-free media (e.g. stem cells) ? I mean some that technique that would also synchronize the cells and/or reduce background signaling noise from media components.
(I once tried withdrawing supplement and growth factors but then my suspension cells turned strangely semi-adherent and began to look sick.)
Maybe try growing your cells in defined medium? Serum-free alternatives commercially available?
Or if you're just synchronising your cells (i presume to keep them in the same cell cycle at G0-G1)? You only have to do that for 24-72h depending on cell type. Then switch it back to serum-containing media. Or you can try chemically-based synchronisation (e.g. roscovitine, aphidicolin, and colchicine) in the presence of serum.