Hello ! it is me again with another question!
I was working on a sample to test a particular treatment on bacteria over 48 hours using crystal violet assay.
After 24 hours, the sample was stained and measured at 595 nm.
By mistake, I did the 48 hours measurements of the sample at 570 nm!!! I am supposed to repeat that to be able to compare the effect after 24 hours vs 48 hours??? how significant could be the resulting OD when I changed the wavelength? any idea? I am googling but no success yet.
Appreciate your input...I hope there is a way to avoid repeating the experiment.