i cultured some cocci on an agar plate and stored this plate for approximately one week at 4°C. Then, i examined the cells using a light microscope. Where I applied the cells to the object slide, I could observe the following image at 40x magnification.
I don't think that these are cells but debris. But what exactly does this debris consist of? How does it emerge and how to avoid it? Why the debris is at a different layer than my cells (heat fixation)?
In terms of debris reduction, would it be better to culture the bactoeria in liquid medium or not storing the agar plate at 4°C.
Thanks for you responses.
Edited by bioforum, 25 August 2013 - 10:15 PM.