I've got trouble in cloning and sequencing.
I amplied fragment of gene I wanted (PCR) with 2 primers, and then check length of PCR product by gel agarose. I sequence PCR product, with reverse primer. I think forward primer is also sequenced in PCR product, this means sequence of PCR product also have forwand primer's sequence, but not have. Of course, primers design to clone, so they have restriction enzyme site. But PCR product is sequenced without restriction enzyme site, why? I dont explain.
Please help me! Thanks.