started using Western Blots last week and have a question regarding my transfer.
This is what the gel looks before and after transfer to a Nitrocellulose membrane using a semi-dry transfer using "NuPage 2x transfer buffer" with 10% Methanol in it (according to the manufacturers protocol for semi-dry blots). 5min @10V, 45min @20V
Please see the attached coomassie gels. On the one post transfer I have these funny looking bubbles and I am wondering if that is something to do with the transfer.
Also I have noticed there is still quite some protein left on the gel.
I appreciate your help.