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quantitation of proteins in Bioanalyzer?

bioanalyzer protein quantitation

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#1 kacej

kacej

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Posted 22 August 2013 - 02:28 AM

Hello,

 

please does somebody have experience with quatitation of proteins using Bioanalyzer 2100? I use protein 80 kit. The problem is that the results are inaccurate. I have even more than 50% difference within triplicate samples. I tried to use both, proteins in original secretion (urine) and acetone precipitated proteins. Two different people tried to prepare the samples and pipet the chip, so it should not be due to pipetting error. 

Do you have any suggestions or have you faced the same problem?

 

thank you







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