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Sodium bicarbonate addition to GMEM for BSR T7/5 cells


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#1 Curtis

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Posted 21 August 2013 - 10:04 PM

I always add 2.5-3.5 g of sodium bicarbonate to DMEM if I make it from its powder form.

 

Now I need to grow a special kind of BHK21 and I prepared GMEM from its powder form. I'm not sure if I need to add sodium bicarbonate to it or not! Is it necessary? 



#2 bob1

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Posted 22 August 2013 - 01:25 AM

It's a modification of minimal Eagle's medium so it will have a bicarb buffering system too.  It will depend on the supplier as to whether they have added the bicarb to the powder or not.



#3 Curtis

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Posted 22 August 2013 - 02:37 AM

yeah, I just checked at Sigma, it is without sodium bicarbonate. should've added, forgot...!






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