Hello dear members,
I read : Staining adherent cells with crystal violet is simple and easily done. It should be noted that some bound cells may be lost due to the staining and washing steps.
A disadvantage of this method is that it does not allow quantitation of cell number in reference to a standard curve. Changes in OD measure qualitative variation in adhesion rather than the number of bound cells.
My question: I thought CV assay quantify the adhered cells by measuring the solubilized crystal violet following the application of ethanol or acetic acid.
I did not understand the underlined : changes in OD measure qualitative variation ....
COuld you pls share your experience?? I just started to use this method.
N.B. will be posting another question in a different topic.