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mRNA electroporation followed by microRNA transfection


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#1 mahrak

mahrak

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Posted 20 August 2013 - 04:41 AM

hello every body

 

 

I am working mRNA delivery. as a part of the job, I have to try transfecting cells with an Invitrotranscribed mRNA containing target sequences for miR-143. here is my question:

1- Since I am transfecting cells with mRNA and it has a very transient nature, shall I co- transfect mature microRNA ( double stranded from mirvana mimics ,Ambion) at the same time or I'd better first transfect the mRNA and then microrna?

2- what is the best method for transfection of mammlian cells with mRNA?

 

 

deeply appreciate any response ASAP






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