I plan to multiplex 20 samples using the Illumina TruSeq ChIP sample prep kit. Kit A (~ $ 2500) contains adapters for 12 samples, and kit B contains the other 12. However I woud like to make the adapters of kit B myself, as I do not want to spend another $ 2500 on kit B, because I do not need so many samples (both kit would be yielding 96 library preps). Illumina has also published the sequences of their adapters, but I am a little worried of some modifications their adapters potentially have. Has anyone ever made such adapters himself? And how is your experience?
Many thanks for your help!
Edited by crockel, 19 August 2013 - 10:12 PM.