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Will long exposures of DNA to UV (302nm) alter sequencing data?


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4 replies to this topic

#1 Ahrenhase

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Posted 19 August 2013 - 02:10 PM

dideoxynucleotide based sequencing, to be exact?



#2 Glynn

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Posted 19 August 2013 - 04:25 PM

I would think so, yea.  UV (UV-B, to be precise, at 302nm) causes dimers between C's and T's.   This, and also the fact that dimers will change the DNA's shape, might interfere with the sequencing reaction.



#3 phage434

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Posted 20 August 2013 - 09:27 AM

Sequencing results will probably not be affected, although the quality of the sequencing will likely be impacted. It's hard to imagine a way in which you would get a different sequence from UV exposure.



#4 Ahrenhase

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Posted 30 August 2013 - 08:24 AM

Sequencing results will probably not be affected, although the quality of the sequencing will likely be impacted. It's hard to imagine a way in which you would get a different sequence from UV exposure.

Wouldn't a polymerase pop off at the first thymine dimer?



#5 phage434

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Posted 30 August 2013 - 08:41 AM

Perhaps a single polymerase at a single location, but remember that there are billions of such polymerases, all active at the same location at the same time. When a single or few of those fall off, nothing will happen to the sequence read.






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