Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

How can i prevent occurring infection in rats injected glucocorticoids?

Animal model

  • Please log in to reply
1 reply to this topic

#1 Faezeh Sadeghi

Faezeh Sadeghi


  • Members
  • Pip
  • 1 posts

Posted 19 August 2013 - 12:14 PM

I want to induce osteonecrosis of femoral head in wistar rats.
for this purpose;Male wistar rat were injected intravenously on days 0 and 1,with 0.4 mg/kg LPS(Sigma-L2880) and intramusculary with 20 mg/kg methylprednisolone acetate on days 3,4,5. After injection of LPS,rats have epistaxis,mouth bleeding,hematuria and hemorrhage in several organs.  few days(approximately 5 days)after last injection of methylprednisolone  rats died due to severe infections.the mortality rate was approximately up to 100%!!!!
My question is how can i prevent occurring of infections???
anybody knows about special conditions for high dose glucocorticoid treatment???
the rats should be kept under sterile condition after MPLS injection???
any suggestion??
we don't have equipment for keeping them under sterile condition in our lab.any suggestion??? 

#2 Fungus_Dreams



  • Active Members
  • PipPipPipPipPip
  • 37 posts

Posted 29 August 2013 - 04:08 PM

Could you have ordered a bad batch of LPS?


Is there a different protocol for administering the LPS? Can you inject it locally? Intrabone?

Also tagged with one or more of these keywords: Animal model

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.