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Cytokine mRNA quantification by Sybr green real-time PCR

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#1 elina_2486



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Posted 18 August 2013 - 10:02 PM

 I am isolating RNA from whole blood and tissue samples by using QIamp Viral RNA extraction kit for determining the relative expression of porcine cytokines TNF-alpha, IFN-gamma, IL-6 & IL-10. Is it necessary to separate PBMC to isolate RNA or blood & tissue samples just work fine for cytokine level estimation by SYBR Green Real Time PCR???????? PLZ HELP........



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