I've tried to extract RNA from the human jaw bone, very hard and minimal amount of cells, The yields were very low (the best RIN got from the bioanalyzer was about 4/10 ). I submerged the samples in RNAlater then used the cryogenic grinder for griding the bone particle, it took about 10 minutes per 3 samples for transfering the samples between the miller tubes. The RNA isolation kit was Qiagen RNeasy lipid tissue kit with the spin columns. Could anyone tell me how to improve the RIN to be good enough for Microarray or qPCR ?
Would it be better if I used TRIZOL with Purelink rna kit ?
Thank you in advance,