If I wish to examine phosphoprotein levels in plasma samples using Western blot, do I just use the samples directly for the experiment like protein lysates? Are there any preparations or taboos I should be aware of?
Western blot using blood plasma
Posted 18 August 2013 - 07:00 PM
Will the use of albumin depletion kit do the job? A reference paper warned that there might be non-specific loss which may affect the analysis.
Posted 28 August 2013 - 09:27 PM
If I performed immunoprecipitation before western blot, will it decrease the non-specific signals? if so, can I directly use the plasma for pull-down? The depletion of albumin should be done BEFORE the immunoprecipitation, right?