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Western blot using blood plasma


Best Answer mdfenko, 16 August 2013 - 02:48 PM

you may find that you have to deplete albumin to get a good look at all of the phosphoproteins.

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#1 polar41323

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Posted 15 August 2013 - 09:22 PM

If I wish to examine phosphoprotein levels in plasma samples using Western blot, do I just use the samples directly for the experiment like protein lysates? Are there any preparations or taboos I should be aware of?



#2 mdfenko

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Posted 16 August 2013 - 02:48 PM   Best Answer

you may find that you have to deplete albumin to get a good look at all of the phosphoproteins.


talent does what it can
genius does what it must
i do what i get paid to do

#3 polar41323

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Posted 18 August 2013 - 07:00 PM

Will the use of albumin depletion kit do the job? A reference paper warned that there might be non-specific loss which may affect the analysis.



#4 mdfenko

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Posted 19 August 2013 - 08:20 PM

there are some albumin depletion kits that are very specific for albumin (such as this one from sigma).


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#5 polar41323

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Posted 19 August 2013 - 08:52 PM

This is very helpful!! Thank you!!



#6 polar41323

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Posted 28 August 2013 - 09:27 PM

If I performed immunoprecipitation before western blot, will it decrease the non-specific signals? if so, can I directly use the plasma for pull-down? The depletion of albumin should be done BEFORE the immunoprecipitation, right?






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