I'm having trouble transfecting HT29 cells. I've been using Hiperfect and also testing a new transfection reagent called Viromer. I need to see gene knockdown after 72 hours. I've seen knockdown at 24hrs using 5nM siRNA, but not at 72hr. If I increase the siRNA to 15nM and 30nM I also see nothing (transfection reagent the same concentration). I'm having no problem at all with DLD1 cells.
I have some Lipofectamine I can use but I've done so many trail and error tests that it's feeling a waste of time!
Has anyone got a protocol at all - even for a different transfection reagent? Or any ideas?
Edited by Elioelio, 15 August 2013 - 04:27 AM.