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siRNA transfection in HT29 cells using Hiperfect or Lipofectamine

HT29 siRNA Transfection Hiperfect Lipofectamine

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#1 Elioelio

Elioelio

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Posted 15 August 2013 - 04:26 AM

I'm having trouble transfecting HT29 cells. I've been using Hiperfect and also testing a new transfection reagent called Viromer. I need to see gene knockdown after 72 hours. I've seen knockdown at 24hrs using 5nM siRNA, but not at 72hr. If I increase the siRNA to 15nM and 30nM I also see nothing (transfection reagent the same concentration). I'm having no problem at all with DLD1 cells.

 

I have some Lipofectamine I can use but I've done so many trail and error tests that it's feeling a waste of time!

 

Has anyone got a protocol at all - even for a different transfection reagent? Or any ideas?

Thanks


Edited by Elioelio, 15 August 2013 - 04:27 AM.






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