I am doing an immunofluorescence experiment on formalin fixed tissue. My normal protocol calls for doing the blocking and antibody incubation steps with Phosphate Buffered Saline + Tween-20, but I mistakenly used regular PBS. At this point, I have carried out blocking and primary incubation with regular PBS instead of PBST; the slide has not been treated with fluorescent secondary antibodies yet, and is currently washing in PBS. How much damage have I done to my experiment? Is it possible to rescue it and get a good result, perhaps by doing an extensive wash to remove nonspecifically bound primary antibody?
Thanks for your time and assistance.
Edited by smlprdd, 14 August 2013 - 02:54 PM.