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PelB Leader Sequence Cleavage


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#1 JamASharpe

JamASharpe

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Posted 13 August 2013 - 01:20 PM

I have designed a fusion peptide that is being inserted into pET22b at the MscI restriction site.  It would appear that the PelB signal peptide cleavage site has been removed in my design process.  What is the consequence of not removing the leader sequence in protein expression?

 

If it is to be a problem then I would like to know as soon as possible so I can by a new primer and add the necessary sequence back into my DNA insert.

 

Thanks in advance.

 

James.


Edited by JamASharpe, 13 August 2013 - 01:20 PM.


#2 JamASharpe

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Posted 13 August 2013 - 01:24 PM

I should add that it is a peptide designed to span the periplasmic membrane.






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