hello to all,
I am planning a methylation study but very new to the subject. I will do gene specific methylation study. I used methprimer (http://www.urogene.org/methprimer/) , I took the first 4000bp region of ABCA1 gene and methprimer gave me 7 CpG islands in the region. Enclosed, you can find the region I put in methprimer.
Is it OK to if I take only promoter region for a methylation study or do I have to look for CpG islands in whole gene?
Do I have to build primers for all CpG islands seperately?
The primer suggestions in methprimer spans only 1st and 2nd islands,why is it so?