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Difference between PFA, acetone and methanol in cell fixation



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#1 science noob

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Posted 12 August 2013 - 05:57 PM

Does anyone know what are the differences between these fixatives? I've tried looking it up on Google but couldn't find a comprehensive guide on this.  Some people use methanol to fix their cells for certain antibodies but others use PFA.



#2 El Crazy Xabi

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Posted 13 August 2013 - 04:52 PM   Best Answer

http://www.abcam.com...ource&rid=11462

 

And more info about mechanisms of aldehides fixation reactions:
http://publish.uwo.c...an/formglut.htm






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