I am having problem with running proteins samples on SDS-PAGE. I don't know the cause but I can show you the problem. I am uploading the pictures of the problem. I read earlier about how to troubleshoot SDS-PAGE. I have tried new Sample buffer and lower the APS and TEMED conc. for stacking gel I have also tried not to screw the plates tightly but nothing worked. First picture is just at the start of the SDS-PAGE and second one of the same PAGE after a while.
I would highly appreciate if someone could explain the problem to me. Its really messing up my experiments.