Hello, I am making DNA probes for a Northern blot (which I have never done before) and have a question:
I am planning to make DNA probes from a PCR product, and label them with a biotin labeling kit (http://www.thermosci...a-labeling-kit/)
Do I need to run the PCR product out on a gel first to gel purify before I label? When I do this PCR reaction it is very clean (can't see any other bands than the one I want), so it doesn't seem necessary, but most protocols I have seen include the gel purify step (unless it is to remove the taq/PCR primers/etc?)
Any other advice or tips from people who have done it this way?