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RNA Immunoprecipitation

RNA IP immuno

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#1 hbm

hbm

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Posted 08 August 2013 - 10:45 AM

I am wondering if anyone has had successful RIPs using mammalian cells stored at -80? I currently have crosslinked, washed, pelleted cells at -80, but they have been there for 2 years. They have not been lysed yet. I would love to use them in future RIPs. Do you think it's worth trying?

 

Also, could anyone recommend of a good RIP kit that's available?






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