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Cleaning probes with 1% DEPC water.


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#1 Wek

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Posted 08 August 2013 - 10:30 AM

I'm cleaning so reusable probes that I use for RNA homogenization. I have looked at a few online guides about incubating the probes with 1% DEPC water but I am confused about the steps.

 

I incubated my probes wih 70% ethanol first and now I am incubating the probes in 1% DEPC water. For the DEPC step I just added the DEPC to water and submerged my probes in it. I'll incubate them over the weekend and then autoclave the probes that are submerged in DEPC water with a Liq20 cycle. 

 

Is there anything wrong with those steps?

 

Thanks


Edited by Wek, 08 August 2013 - 10:31 AM.


#2 bob1

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Posted 08 August 2013 - 12:45 PM

Sounds fine to me, though make sure the probes can stand the autoclaving first...



#3 Wek

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Posted 10 August 2013 - 07:04 AM

Great, I have never done this before so I wasn't sure. Btw, would I be able to remove the probes from the DEPC water, wrap them in aluminum foil, and autoclave them? Or do I need to autoclave the probes while they are in the DEPC water?



#4 phage434

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Posted 10 August 2013 - 07:19 AM

The RNAse destruction is done by the DEPC, not by the autoclaving. The autoclave treatment is to remove the DEPC. So, it should be fine to wrap and autoclave.






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