I am running some long agarose gels to be used for subsequent blotting for a Southern hybridization. So far it was so good and suddenly I found my gel start shrinking from the top after running for about 6 hours at 40-60V. What could be the reason?
I am using 0.8% agarose gels to run restriction enzyme digested genomic DNA with 1% TAE buffer. Each time I am using new buffer. I tried with different gel boxes as well as different power supplies. I checked the buffer level too and it was well adequate to cover the gel entirely (that means no gel dehydration) but I couldnot find where I did wrong????