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which antigen should I choose for +ctrl?


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#1 joy123

joy123

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Posted 07 August 2013 - 08:40 AM

Hi, I am looking for a proper antigen for PBMCs.

 

In the patient group, "CD4+ and CD8+ αβ T cells and γδ T cells" are supposed to be activated in PBMCs unders stimulation. So, I need to find some antigen to activate T cells in healthy controls' PBMCs in a similar way (or as close as possible).

 

I am thinking about using some global T cell antigens. Maybe PMA, PHA or CD3/CD28?

 

Do you have any suggestions? I appreciate a lot!

 

Thanks!






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