Hi, I am looking for a proper antigen for PBMCs.
In the patient group, "CD4+ and CD8+ αβ T cells and γδ T cells" are supposed to be activated in PBMCs unders stimulation. So, I need to find some antigen to activate T cells in healthy controls' PBMCs in a similar way (or as close as possible).
I am thinking about using some global T cell antigens. Maybe PMA, PHA or CD3/CD28?
Do you have any suggestions? I appreciate a lot!