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# Diluted Sample OD

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### #1 Inbox

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Posted 06 August 2013 - 11:12 PM

Undiluted Solution has OD of >1 and after diluting two times OD is 0.6. how to calculate OD of undiluted Sample?

Is it 0.6 X 2 = 1.2 ? ( Multiplied OD by Dilution Factor)

thanks.

### #2 pcrman

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Posted 06 August 2013 - 11:30 PM

I think your original OD should be 2.4 assuming your dilution is 1:1.  After first dilution, you get 1.2, and 0.6 the 2nd time.

the formula is 0.6/0.5/0.5 = 2.4

### #3 newborn

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Posted 07 August 2013 - 05:19 AM

May be he meaned that he diluted it at 1:2. if that was the case 1.2 was correct.

### #4 Inbox

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Posted 08 August 2013 - 12:02 PM

Thank you both. I did diluted 1:2