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Methylight Results Analysis

methylight epigenetic qmsp dna methylation

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#1 Nyquist

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Posted 06 August 2013 - 03:02 PM

Hi, I'm doing the methylation analysis of two genes, using ACTB as a normalizer, and a 100% methylated DNA as positive control, I have made the bisulfite convertion, and a standard curve with the positive control, I run my DNA samples (previously bisulfite modified) and now, I don't know how to analyse the results. I know that I have to calculate a ratio between the quantitiy of methylated DNA of my target gene and beta actine gene, but, how I do it?, I use logarithmic data?, or I just do it and them I apply log?, because the majority of the figures of methylation the use type of samples (X) vs log of methylation ratio, anyone know?, please help me.

 

René



#2 Nyquist

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Posted 06 August 2013 - 03:14 PM

Hi, I'm doing the methylation analysis of two genes, using ACTB as a normalizer, and a 100% methylated DNA as positive control, I have made the bisulfite convertion, and a standard curve with the positive control, I run my DNA samples (previously bisulfite modified) and now, I don't know how to analyse the results. I know that I have to calculate a ratio between the quantitiy of methylated DNA of my target gene and beta actine gene, but, how I do it?, I use logarithmic data?, or I just do it and them I apply log?, because the majority of the figures of methylation the use type of samples (X) vs log of methylation ratio, anyone know?, please help me.

 

René







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