Recently i did qpcr and in this i found the CT value of GAPDH reaching 26.Its the first time i got a value like this. Most of the time i get at 18 or 19 Ct but now its around 26.
Ct value of the sample is at 28.
Do you think that there is some thing wrong?Could anyone please tell me why it is like this. If so does it affect while doing ablsoute quantification.
find the attached picture
thanks in advance