When I run my pcr product (recombinant plasmid as template with insert primers) in 2% agarose gel (settings: 90V for 70min) I get these wavy lines. These lines I suspect are due to an incorrectly made gel, but I have searched for and tried many gel making protocols and still get these wavy bands; so, I am beginning to think maybe the product itself is somehow leading to this appearance.
However, I have rerun the product that initially resulted in wavy lines in gel lanes that have been run with positive control products and show straight bands, and sometimes get straight lines and sometimes get wavy lines, which leads me to believe that it indeed isn't the product itself but the gel or perhaps the settings that are leading to the wavy bands.
Can someone please let me know what this phenomenon is the result of?
P.S. image attached
Edited by tihong10, 06 August 2013 - 05:24 AM.