I have no experience on kinase or phosphorylation but plan to do experiment on this field.
I am planing to check the interaction of candidate phosphoproteins with my target protein. So I would like to produce phosphorylated proteins in E.coli. If I know only what the protein is, the site of phosphorylation, is it possible to guess what kind of kinase is used for the phosphorylated protein? and I know the state of cell that express the phosphorylated proteins.
If it is possible. Do you think which way is better for phosphorylation between co-expression both proteins in E.coli, or produce kinase proteins separately and perform in vitro phosphorylation between 2 proteins.
Thank you in advance for suggestion.