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ovarian cancer cell line: phenotype disappears


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#1 bongiwoman

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Posted 05 August 2013 - 02:16 PM

Hello there,
Does anybody know what can cause the disappearance of a phenotype in cell lines? I am knocking down transporter genes in various ovarian cancer cell lines and it already happened twice that a phenotype (in colony formation assays) disappeared (I had fewer colonies in the knockdowns but later there was no difference anymore). Also, one of the cell lines had in general a lot more colonies than before and they also looked a bit different. Does anyone have an advice for me? By the way, the passage numbers were only a few passages apart.
Thanks, Angelika

#2 bob1

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Posted 02 April 2015 - 12:27 AM

Hmmm... mycoplasma?



#3 bongiwoman

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Posted 14 May 2015 - 09:25 PM

No mycoplasma, bob1, but thanks for the reply! I am thinking if it might be off target effects?? Do you think this could be possible? Thanks!



#4 bob1

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Posted 15 May 2015 - 12:02 AM

Off target effects are very common for knockdown using siRNA/miRNA/shRNA  mediated KD, so it is entirely possible that this is the cause of what you are seeing.






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