Posted 12 August 2013 - 09:28 AM
Correct me if I am wrong, My impression is you are using Anti-His for your WB detection? Or do you mean you are using specific some antibody?
Just curious, can you detail to us your primary and secondary antibody origins?
Based on what you mentioned, I am thinking the possibility of your recombinant protein might be forming a complex with something else. Possible for you to isolate the two proteins and perform mass spec analysis?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434