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Cost of Sequencing by Post


Best Answer pito, 02 August 2013 - 09:00 AM

Well.... one important "detail" is missing: what are you sequencing? (whole genome? Insert in a plasmid....?)

 

Without this information its almost impossible to state how much it would cost.

 

For example: sequencing an insert in a known plasmid vector can be done for +- 5 us dollar.

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#1 centralamgod

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Posted 02 August 2013 - 05:52 AM

Hello all,

 

I have a question for those of you who send their samples away for DNA sequencing.  Whom are you using, and what does it roughly cost?

 

Thanks in advance.



#2 pito

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Posted 02 August 2013 - 09:00 AM   Best Answer

Well.... one important "detail" is missing: what are you sequencing? (whole genome? Insert in a plasmid....?)

 

Without this information its almost impossible to state how much it would cost.

 

For example: sequencing an insert in a known plasmid vector can be done for +- 5 us dollar.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 pcrman

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Posted 02 August 2013 - 09:27 AM

I agree with pito, cost/per run for sequencing pcr products, plasmids is around $5 USD nowadays.

#4 centralamgod

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Posted 02 August 2013 - 09:30 AM

Sorry,

 

I'm sequencing plasmid DNA



#5 pito

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Posted 03 August 2013 - 11:22 AM

Sorry,

 

I'm sequencing plasmid DNA

Insert in a known plasmid or a entire plasmid? (new plasmid? known plasmid with some adaptation...? )

 

Normally it should not cost too much.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#6 doxorubicin

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Posted 04 August 2013 - 11:28 AM

For standard DNA sequencing:  We pay 4,40 EUR for prepaid barcodes and 3,90 EUR per sample for 96-well plates from Eurofins.  In the US, Genscript is probably quite inexpensive: http://www.genscript...ml?src=pullmenu



#7 centralamgod

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Posted 05 August 2013 - 06:33 AM

Thanks for all of the info.  It is a known plasmid with no adaptiations.



#8 pito

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Posted 05 August 2013 - 06:39 AM

Thanks for all of the info.  It is a known plasmid with no adaptiations.

Well, like said .. 5 dollars will do the trick..
And to find a company: just google it... there are many companies out there and I have no idea what would be the best for you..

(where do you live for example...? what exactelly is it that you need.. dont be afraid to contact companies and ask them... some will even offer special prices for new users...)

And think about the big players out there too.. they are often very cheap too and can do it fast...

 


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#9 memari

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Posted 05 August 2013 - 07:33 PM

http://www.mwg-biotech.com/

 

www.operon.com


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Babak Memari




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