I have to calculate the dilution factor of serum in my preparation to be able to calculate the final concentration in a HPLC setup.
This is the prep:
25 ul serum mixed with 5 ul solvent + 1000 ul AcN (to precipitate proteins)
pippette 995 ul into new epp. and evaporate to dryness in speedvac
reconstitute with 100 ul solvent
How does one calculate the dilution factor??