Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Calculation of a dilution factor??

dilution serum factor HPLC

Best Answer mdfenko, 01 August 2013 - 04:49 AM

for your purpose, the initial dilution is not important (25 ->1030 ul (=41.2x)) other than for the determination of what fraction of the starting material is dried (995/1030=0.966) and reconstituted.

 

100/(25*0.966)=4.14x diluted

Go to the full post


  • Please log in to reply
2 replies to this topic

#1 esbena

esbena

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 01 August 2013 - 03:06 AM

Hi all.

One question...

I have to calculate the dilution factor of serum in my preparation to be able to calculate the final concentration in a HPLC setup.

This is the prep:

25 ul serum mixed with 5 ul solvent + 1000 ul AcN (to precipitate proteins)

vortex

pippette 995 ul into new epp. and evaporate to dryness in speedvac

reconstitute with 100 ul solvent

How does one calculate the dilution factor??
 
Can´t wrap my head around this one...

Thanks


#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 01 August 2013 - 04:49 AM   Best Answer

for your purpose, the initial dilution is not important (25 ->1030 ul (=41.2x)) other than for the determination of what fraction of the starting material is dried (995/1030=0.966) and reconstituted.

 

100/(25*0.966)=4.14x diluted


talent does what it can
genius does what it must
i do what i get paid to do

#3 esbena

esbena

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 01 August 2013 - 05:13 AM

Thanks







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.