I have started with my protein work to confirm some mRNA expression results with Western Blots.
When I extracted the RNA from 25mg small intestine using a Qiagen RNeasy Lipid tissue kit I kept the non-RNA phases and I now isolated the protein fraction with the "Isolation of genomic DNA and/or proteins from fatty tissue samples treated with QIAzol™ Lysis Reagent" User developed protocol.
I now presumably got protein in 8M Urea/50mM DTT and analysed that in a Bradford assay (had to establish that aswell in my lab...) and get concentrations of about 0.5 mg/ml; I got about 400ul of that.
I am just wondering if that concentration seems about right? Is there any other way to test if I actually got protein in my samples?
Thanks for your help!