I have been working on a DNA extraction and one of the steps involves to remove the unwanted foreign DNA by destryoing it and destroying bacterial cells in the sample with bleach.
The bleach itself does not damage my cells so its easy to use, however during the bleach step, the protocol states to keep the sample with the bleach on ice. Why is this? Doesnt the bleach work at room temperature?
Or is it just to prevent that the chlorine evaporates and thus making the bleach worthless ?
Or is it also to prevent my cells to be damaged? Which seems weird..
any other ideas?
I was thinking about preventing DNAses to become active.. but they have already been removed by a previous step in which they have been destroyed already.