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Difference between IHC and florescent IHC


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#1 harry348

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Posted 31 July 2013 - 12:15 AM

Hello all,

 

could you please tell me what is the difference between IHC and florescent IHC and at what step?

and the protocol for florescent IHC?

 

thanks in advance

 

harry



#2 bob1

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Posted 31 July 2013 - 12:57 AM

The difference is in the secondary antibody - for fluorescence it is fluorescent (obviously) and for normal IHC it is conjugated to an enzyme to develop a colour based chemical reaction.  The protocols are the same essentially, just swap the secondary for a fluorescent one (work in the dark) and skip the enzymatic reaction step.






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