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What happens if we use expired tissue culture flasks (T-162 Flasks) for vero cel

Expired T-162 flasks

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#1 pavanisharath25

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Posted 29 July 2013 - 03:44 PM

One of my QC technician used expired t-162 flasks for vero cell passage. The cells were looking fine and there was no contamination.

Can any one suggest me how to provide justification.

#2 bob1

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Posted 31 July 2013 - 01:00 AM

The expiry is usually for guaranteed sterility, so most of the time this shouldn't be a problem, so long as the packaging is intact.  Some culture vessels have substances (e.g. gelatin) added that can degrade with time, I wouldn't use that sort past expiry dates.






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