I'm trying to detect very small amount of salmon DNA. I'm using qpcr with primers for two different mitochondrial genes, Cyto oxidase subunit 1 and Cyto b. I'm hitting my limit of detection around 1pg. I'd like to increase it but I'm not sure how. Part of my problem is that I'm not sure how many copies of my genes are present in 1pg of material. I have tried two primer sets for each gene and used both probe detection and two kinds of Sybrgreen.
Any advice? Should I switch genes? Do pre-amplification?