Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Isotype control for multiple CD antigens of interest

Flow cytometry Isotype control antibody isotype

  • Please log in to reply
1 reply to this topic

#1 Tarique

Tarique

    member

  • Active Members
  • Pip
  • 5 posts
2
Neutral

Posted 29 July 2013 - 04:52 AM

I am using multicolor flow cytometry. The number of CD markers of my interest are 6. All of my flow antibodies are IgG isotype from mouse. Can I use only one isotype control of same antibody isotype from same species (e.g., mouse IgG-PE) for multiple antibodies of same isotype and from same species [e.g., for mouse anti-human CD14-FITC (IgG), mouse anti-human CD68-PE (IgG), mouse anti-human CD11b-APC (IgG)]? Buying 6 isotype control is very expensive!!!



#2 Tabaluga

Tabaluga

    Making glass out of shards

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 403 posts
49
Excellent

Posted 29 July 2013 - 06:13 AM

It's really expensive... But the problem is that they are all different colours. So if you use only mouse IgG-PE, this actually will tell you nothing about the "control fluorescence level" in the FITC or APC Channel. It only gives you the level for the PE staining. Another thing to consider is concentrations. The isotype control should be applied in the same amount as the antibody itself. But the main problem here really is the different colours. And using unstained controls, would that be an option ?


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.