attached you will find protein gel I just made and I have some problems to fix:
You see that the protein lanes get first thinner and second more blurry the smaller the proteins are.I have no idea how to fix that?
I've read some threads saying salt concentrations might be an issue. Right now, I'm dialysing my samples to see if it helps, but i do you think it will prevent that the proteins lines become thinner?
I used a discontinuos SDS-PAGE gel with 12.5%, the samples are a whole cell extract from a bacterial culture (B. subtilis)
Thank you in advance for any help I can get!