Doubt: genomic dna library construction
Posted 25 July 2013 - 12:07 PM
while constructing a genomic dna library.. can it be ensured that one clone recieves only plasmid?
what i mean is.. after ligating the digested genomic dna to a suitable vector.. when it is transformed into E. coli.. what is the guarantee that two plasmids with two different inserts will not enter the same E. coli???
hope some one can help me with this
Posted 25 July 2013 - 12:26 PM
Posted 26 July 2013 - 12:54 AM
will there be no incompatibility factor that won't allow two different plasmids entering the same cell?
Posted 27 July 2013 - 01:36 PM
There could be incompatiblity, but the chances are relatively low.