Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Doubt: genomic dna library construction


  • Please log in to reply
3 replies to this topic

#1 susnata

susnata

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 25 July 2013 - 12:07 PM

i just had this doubt..

while constructing a genomic dna library.. can it be ensured that one clone recieves only plasmid?

what i mean is.. after ligating the digested genomic dna to a suitable vector.. when it is transformed into E. coli.. what is the guarantee that two plasmids with two different inserts will not enter the same E. coli???

hope some one can help me with this :)

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,666 posts
396
Excellent

Posted 25 July 2013 - 12:26 PM

There's no guarantee, but it is possible to screen for different inserts...

#3 susnata

susnata

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 26 July 2013 - 12:54 AM

well.. so it would be a wrong to make a statement that... "each colony observed represents a single clone".. they can represent two different cloned plasmids aswell??

will there be no incompatibility factor that won't allow two different plasmids entering the same cell?

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,666 posts
396
Excellent

Posted 27 July 2013 - 01:36 PM

There could be incompatiblity, but the chances are relatively low. 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.