How accurate is nanodropping?nanodrop DNA measure
Posted 23 July 2013 - 03:31 PM
I mix the sample very well before nanodropping it. The sample is plasmid DNA from a Qiagen spin miniprep. This isn't the first time this has happened to me with nanodropping. I've read that it can be inaccurate, but never by that amount.
Posted 23 July 2013 - 03:46 PM
Posted 23 July 2013 - 07:01 PM
Posted 24 July 2013 - 01:33 AM
I also agree with phage434 that it's better to use 1.5 or 2 µl aliqouts.
Posted 24 July 2013 - 06:22 AM
It is just for making easy the process of reading of OD of more than 4 samples.
There is another machine that claims they removed the movable pedestal of NanoDrop for more accuracy.
Posted 24 July 2013 - 11:20 AM