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penicillin/streptomycin in cell culture


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#1 Angelika

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Posted 23 July 2013 - 07:44 AM

Hi
Does penicillin/ strepromycin go off after some time after being using to prepare cell culture media and stored in the fridge/ used at 37 every now and then?
I will be grateful for your help
Angelika

#2 bob1

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Posted 23 July 2013 - 12:34 PM

Yes it degrades over time.

#3 Angelika

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Posted 23 July 2013 - 11:47 PM

Thank you. After how much time does it need to be replaced?

#4 bob1

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Posted 24 July 2013 - 01:12 AM

The period is dependent on the time at room temp/37 and light exposure.

In most instances (exceptions are selection for an introduced marker and primary cultures), you shouldn't need antibiotics in your cultures if your sterile technique is adequate.




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